WIDAL TEST (O & H ANTIGENS)

The WIDAL Test (O & H Antigens) is a serological diagnostic test used for the detection of enteric fever (typhoid and paratyphoid fevers) caused by Salmonella typhi and Salmonella paratyphi. The test detects agglutinating antibodies (O = somatic antigen, H = flagellar antigen) present in a patient’s serum.

Type: Slide or tube agglutination test

Reagents: Contains standardized suspensions of Salmonella typhi O and H antigens (sometimes includes S. paratyphi AH, BH antigens as well).

Sample Required: Patient’s serum

Result Time: Within minutes (slide method) or few hours (tube method)

Key Features:

Detects antibodies to Salmonella typhi (O & H antigens)

Simple, rapid, and cost-effective diagnostic method

Suitable for both screening and confirmation

Easy-to-use reagents with clear agglutination visibility

Commonly available in 5 ml, 10 ml, or kit form

Uses:

Diagnosis of typhoid and paratyphoid fever

Screening test in pathology labs, hospitals, and diagnostic centers

Helps clinicians in early diagnosis and treatment planning